Interested in promotions? | Click here >>
8669
SignalKine™ Human IL-4 Sandwich ELISA Kit
ELISA Kits

SignalKine™ Human IL-4 Sandwich ELISA Kit #8669

Reviews ()
Citations (0)

Sample Testing

To Purchase # 8669

Important Ordering Details

Custom Ordering Details: Product is assembled to order. Please allow up to three business days for your order to be processed.

Supporting Data

REACTIVITY H

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

SignalKine™ Human IL-4 Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-4 (hIL-4) in multiple matrices. Unknown samples being tested for hIL-4 and hIL-4 standards are added to low volume microwells, where the hIL4 is captured by the coated hIL-4 Rabbit mAb. Following a washing step, a biotinylated hIL-4 Detection Rabbit mAb is added to detect the captured hIL-4. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-4 Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hIL-4 in the sample.

SignalKine™ Human IL-4 Sandwich ELISA Kit detects hIL-4 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-4 protein standard provided. The hIL-4 standard range is from 15.6 to 1000 pg/ ml. Samples containing higher levels of hIL-4 can be diluted to fit into the standards range.

Specificity / Sensitivity

Species Reactivity:

Human

Background

Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).

  1. Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
  2. Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
  3. Kopf, M. et al. (1993) Nature 362, 245-8.
  4. Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
  5. Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
  6. Hou, J. et al. (1994) Science 265, 1701-1706.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.