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7898
SignalKine™ Human IL-8 Sandwich ELISA Kit
ELISA Kits

SignalKine™ Human IL-8 Sandwich ELISA Kit #7898

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Figure 1. PBMC supernatants, untreated (-) or PHA-treated (10 µg/ml; +) were removed after 1 and 5 d. hIL-8 levels in the supernatants were quantified using SignalKine™ Human IL-8 Sandwich ELISA Kit.

Typical Data. Human IL-8 Standard Curves, one diluted in SignalKine™ Sample Diluent S01, a second diluted in

SignalKine™ Sample Diluent S02 and a third in cell culture media (RPMI + 10% FBS). Although these are typical of the standard curves that will be generated using this kit, a new set of standards should be run for each new experiment.

To Purchase # 7898

Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Supporting Data

REACTIVITY H

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

SignalKine™ Human IL-8 Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-8 (hIL-8) in multiple matrices. An hIL-8 Rabbit mAb has been coated onto low volume microwells. hIL-8 standards and hIL-8-containing samples are added to the wells and captured by the coated antibody. Following a washing step, a biotinylated hIL-8 Detection Rabbit mAb is added to detect the captured hIL-8. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-8 Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hIL-8 in the sample.

SignalKine™ Human IL-8 Sandwich ELISA Kit detects hIL-8 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-8 protein standard provided. The hIL-8 standard range is from 15.6 to 1000 pg/ml. Samples containing higher levels of hIL-8 can be diluted to fit into the standard range.

Specificity / Sensitivity

Species Reactivity:

Human

Background

IL-8 is the prototypical CXC chemokine. It is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1,2,3). IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct G protein-coupled receptors for IL-8, CXCR1 and CXCR2 (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

  1. Payne, A.S. and Cornelius, L.A. (2002) J Invest Dermatol 118, 915-22.
  2. Brat, D.J. et al. (2005) Neuro Oncol 7, 122-33.
  3. Mukaida, N. (2003) Am J Physiol Lung Cell Mol Physiol 284, L566-77.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.