|1 Kit (96 assays)
|H M R Mk
|4E-BP1 (Thr37/Thr46) Rabbit mAb Coated Microwells
|4E-BP1 Mouse Detection mAb
|Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated)
|Detection Antibody Diluent
|TMB Substrate 7004
|STOP Solution 7002
|ELISA Wash Buffer (20X) 9801
|ELISA Sample Diluent
|Cell Lysis Buffer (10X) 9803
NOTE: Prepare solutions with purified water.
*NOTE: Some PathScan® ELISA Kits may include HRP-Linked Streptavidin in place of HRP-Linked Antibody.
NOTE: Initial color of positive reaction is blue, which changes to yellow upon addition of STOP Solution.
posted November 2013
Protocol Id: 204
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
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