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  1. Products /
  2. ELISA Kits /
  3. PathScan® Phospho-eIF2 alpha (Ser51) Sandwich ELISA Kit

PathScan® Phospho-eIF2 alpha (Ser51) Sandwich ELISA Kit #7948

Inquiry Info. # 7948

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    Product Specifications

    REACTIVITY

    Product Information

    Product Description

    The PathScan® Phospho-eIF2α (Ser51) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eIF2α when phosphorylated at Ser51. A eIF2α rabbit antibody has been coated onto the microwells. After incubation with cell lysates, eIF2α protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a biotinylated phospho-eIF2α (Ser51) detection antibody is added to detect captured eIF2α protein phosphorylated at Ser51. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of eIF2α phosphorylated at Ser51.

    Specificity / Sensitivity

    PathScan® Phospho-eIF2 alpha (Ser51) Sandwich ELISA Kit #7948 detects endogenous levels of eIF2α protein only when phosphorylated at Ser51 (see Figure 1). This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Background

    Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

    Alternate Names

    EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; eIF2-alpha; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; IF2A

    Database Links

    UniProt ID: P05198


    Entrez-Gene Id: 1965


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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