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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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REACTIVITY
Product Includes Volume Cap Color
HER2/ErbB2 Capture Ab(100X) 0.4 ml Pink
HER2/ErbB2 (Y1221/ 1222) Detection Ab (100X) 0.4 ml Blue
Anti-rabbit IgG, HRP-linked Antibody 0.04 ml Red

Product Description

CST's PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Kit #7148. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The HER2/ErbB2 Capture Antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by a Phospho-HER2/ErbB2 (Tyr1221/1222) Detection Antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-HER2/ErbB2 (Tyr1221/1222) protein.

Antibodies in kit are custom formulations specific to kit.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).


1.  Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.

2.  Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.

3.  Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.

4.  Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.

5.  Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.


Entrez-Gene Id 2064
Swiss-Prot Acc. P04626


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

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PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Antibody Pair