|Product Includes||Volume||Cap Color|
|IRS-2 Capture Ab (100X)||0.4 ml||Pink|
|P-Tyrosine Detection Ab (100X)||0.4 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody (1000X)||0.04 ml||Yellow|
CST's PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-Tyrosine Mouse Detection Antibody and an HRP-conjugated, Anti-Mouse IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2 phosphorylated on tyrosine.
Antibodies in kit are custom formulations specific to kit.
Antibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily.
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PathScan is a trademark of Cell Signaling Technology, Inc.
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