|Product Includes||Volume||Cap Color|
|LAT Capture Ab (100X)||0.4 ml||Pink|
|P-LAT (Y191) Detection Ab (100X)||0.4 ml||Blue|
|Anti-rabbit IgG, HRP-linked Antibody||0.04 ml||Red|
CST's PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Kit #7936. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The LAT mouse capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a phospho-LAT (Tyr191) rabbit detection antibody and HRP-conjugated, anti-rabbit IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-LAT (Tyr191).
Antibodies in kit are custom formulations specific to kit.
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PathScan is a trademark of Cell Signaling Technology, Inc.
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