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  3. PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair

PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair #7937

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  • ELISA

Inquiry Info. # 7937

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Storage

    Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

    Protocol

    Product Description

    CST's PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Kit #7936. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The LAT mouse capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a phospho-LAT (Tyr191) rabbit detection antibody and HRP-conjugated, anti-rabbit IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-LAT (Tyr191).
    Antibodies in kit are custom formulations specific to kit.

    Specificity / Sensitivity

    For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

    Alternate Names

    antigen presentation; CD3; cell based assay; lymphocyte; lymphocyte activation; sandwich ELISA; screening; T cell; T cell activation; T cell receptor; zap70

    Database Links

    UniProt ID: O43561


    Entrez-Gene Id: 27040


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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