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Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-11-20 16:46:07.291
Product last modified at: 2024-11-21T08:01:09.232Z
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PDP - Template Name: ELISA Antibody Pair
PDP - Template ID: *******c8d7b7a

PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair #7963

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  • ELISA

Important Ordering Details

Custom Ordering Details: Product is assembled to order. Please allow up to three business days for your order to be processed.

    Supporting Data

    REACTIVITY H Dg
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • Dg-Dog 

    Product Information

    Storage

    Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

    Protocol

    Product Description

    Cell Signaling Technology's PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit #7953. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-Src (Tyr416) rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a total Src mouse detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Src (Tyr416).
    Antibodies in kit are custom formulations specific to kit.

    Specificity / Sensitivity

    For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Dog

    Background

    The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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