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PhosphoSitePlus® Resource

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Product Includes Volume Solution Color
Phospho-Survivin (Thr34) Rabbit mAb Coated Microwells 96 tests

Product Description

The PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of survivin protein phosphorylated at Thr34. A phospho-survivin (Thr34) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-survivin protein is captured by the coated antibody. Following extensive washing, a survivin mouse antibody is added to detect the captured phospho-survivin protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of survivin phosphorylated at Thr34.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

CST's PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit #7193 detects endogenous levels of survivin protein phosphorylated at Thr34 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).


1.  Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.

2.  Li, F. et al. (1998) Nature 396, 580-584.

3.  Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.

4.  O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.

5.  Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.

6.  Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.


Entrez-Gene Id 332
Swiss-Prot Acc. O15392


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7193
PathScan® Phospho-Survivin (Thr34) Sandwich ELISA Kit