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Render Timestamp: 2024-12-06T10:43:34.142Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-11-25 15:08:07.274
Product last modified at: 2024-09-19T22:12:48.419Z
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PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® Phospho-Tau (Ser214) Sandwich ELISA Kit #44177

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  • ELISA

    Supporting Data

    REACTIVITY H M R
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    The PathScan® Phospho-Tau (Ser214) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tau protein when phosphorylated at Ser214. A phospho-tau (Ser214) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-tau (Ser214) is captured by the coated antibody. Following extensive washing, a tau mouse antibody is added to detect the captured phospho-tau protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of tau protein phosphorylated at Ser214.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® Phospho-Tau (Ser214) Sandwich ELISA Kit detects endogenous levels of tau protein when phosphorylated at Ser214, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse, Rat

    Background

    Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

    Numerous kinases including PKA, CDK5, GSK3β and SGK1 have been shown to phosphorylate tau at Ser214. In addition, investigators have shown that tau is phosphorylated at Ser214 in Alzheimer's disease and dementia with Lewy bodies (4-9). Investigators have shown that tau phosphorylation at Ser214 detaches tau protein from microtubules, protecting it against aggregation into Alzheimer paired helical filaments (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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