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  3. PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair

PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair #7824

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  • ELISA

Inquiry Info. # 7824

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Storage

    Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

    Protocol

    Product Description

    CST's PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit #7335. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The VEGFR-2 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-VEGFR-2 (Tyr1175) detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-VEGFR-2 (Tyr1175) protein.
    Antibodies in kit are custom formulations specific to kit.

    Specificity / Sensitivity

    For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

    Alternate Names

    KDR; VEGF Receptor; vegfr; VEGFR-2 elisa; vegfr2

    Database Links

    UniProt ID: P35968


    Entrez-Gene Id: 3791


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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