PathScan® RP Ikaros Sandwich ELISA Kit #94391
- ELISA
Supporting Data
| REACTIVITY | H M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Ikaros Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Ikaros protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Ikaros in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Ikaros protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Ikaros (IKZF1, LYF1) is the prototypical Ikaros family zinc-finger transcription factor and is expressed abundantly in lymphoid cells. Genetic studies in mice demonstrate that Ikaros is a tumor suppressor that is important for the normal development of B, T, natural killer, and dendritic cells (3,4). Additional studies show that imbalanced expression of different Ikaros isoforms, as well as mutations in the corresponding IKAROS gene, can be associated with a number of hematologic malignancies in humans (2,5,6).
- Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54.
- Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
- Georgopoulos, K. et al. (1997) Annu Rev Immunol 15, 155-76.
- Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25.
- Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35.
- Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.
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