PathScan® RP MG-H1 Glycated Histone H3 (Arg17) Sandwich ELISA Kit #32428
- ELISA+
Important Ordering Details
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Product Specifications
| REACTIVITY | H M |
Application Key:
- ELISA+-ELISA and/or ELISA-like Assays
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Methylglyoxal-derived hydroimidazolone 1 (MG-H1) is the most predominant of three primary methylglyoxal-derived hydroimidazolone isomers (MG-H1, MG-H2, and MG-H3) that form as advanced glycation end-products when methylglyoxal (MGO) non-enzymatically reacts with arginine residues on proteins.
The PathScan® RP MG-H1 Glycated Histone H3 (Arg17) Sandwich ELISA Kit detects endogenous MGO-derived glycation of the MG-H1 subtype at Arg17 of histone H3. This kit may also weakly detect MG-H2 glycated Arg17 of histone H3. In addition, this kit may weakly detect an additional MG-H1 glycated arginine residue on histone H3. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Background
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Harmel, R. and Fiedler, D. (2018) Nat Chem Biol 14, 244-252.
- Galligan, J.J. et al. (2018) Proc Natl Acad Sci USA 115, 9228-9233.
- Thornalley, P.J. et al. (1999) Biochem J 344 Pt 1, 109-16.
- Maessen, D.E. et al. (2015) Clin Sci (Lond) 128, 839-61.
- Zheng, Q. et al. (2019) Nat Commun 10, 1289.
Alternate Names
H3; H3 clustered histone 1; H3 histone family, member A; H3/A; H31; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; Histone H3; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Limited Uses
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