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PathScan® RP Phospho-TrkA (Tyr674/675) Sandwich ELISA Kit #7212

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  • ELISA

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    Supporting Data

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Phospho-TrkA (Tyr674/675) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects transfected levels of Phospho-TrkA (Tyr674/675) protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with TrkA protein phosphorylated at Tyr674/675 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of TrkA protein phosphorylated at Tyr674/675. Learn more about all of your ELISA kit options here.

    ​​​​​​​*Antibodies in kit are custom formulations specific to kit.
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    Protocol

    Specificity / Sensitivity

    PathScan® RP Phospho-TrkA (Tyr674/675) Sandwich ELISA Kit #7212 detects transfected levels of phospho-TrkA (Tyr674/675) Protein. As shown in Figure 1, using this ELISA Kit #7212, a significant induction of phospho-TrkA (Tyr490) is detected in 3T3/TrkA cells treated with NGF. However, the levels of total TrkA (phospho and nonphospho) in untreated and NGF-treated cells detected by PathScan® RP Total TrkA Sandwich ELISA Kit #7208, remain unchanged. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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