PathScan® RP Phospho-Tyrosine Hydroxylase (Ser31) Sandwich ELISA Kit #55853
- ELISA+
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
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Product Specifications
| REACTIVITY | H R |
Application Key:
- ELISA+-ELISA and/or ELISA-like Assays
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Phospho-Tyrosine Hydroxylase (Ser31) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine hydroxylase protein phosphorylated at Ser31 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-tyrosine hydroxylase (Ser31) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-tyrosine hydroxylase (Ser31). Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
- Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
- Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
- Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
- Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
- Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
- Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
- Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
- Saraf, A. et al. (2007) J Biol Chem 282, 573-80.
Alternate Names
TH; The; TY3H; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase
Limited Uses
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