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PathScan® RP Pyk2 Sandwich ELISA Kit #62171

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  • ELISA+

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    Product Specifications

    REACTIVITY H M
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Pyk2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Pyk2 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Pyk2 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Pyk2 protein. Learn more about your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP Pyk2 Sandwich ELISA Kit detects endogenous levels of Pyk2 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    Protein-tyrosine kinase 2 (Pyk2), also called CAKβ, RAFTK, and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).

    Alternate Names

    CADTK; CAK beta; CAK-beta; CAKB; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2; FAK2; Focal adhesion kinase 2; PKB; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; Related adhesion focal tyrosine kinase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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