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  3. PathScan® RP TMEM106B Sandwich ELISA Kit

PathScan® RP TMEM106B Sandwich ELISA Kit #35127

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  • ELISA

Important Ordering Details

Custom Ordering Details:

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    Product Specifications

    REACTIVITY H M R Mk
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP TMEM106B Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of TMEM106B protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with TMEM106B in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of TMEM106B protein. Learn more about your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP TMEM106B Sandwich ELISA Kit detects endogenous levels of TMEM106B protein. This ELISA kit detects full-length TMEM106B as both antibodies used in this kit recognize the N-terminal region of TMEM106B. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Background

    Transmembrane protein 106B (TMEM106B) is a type II transmembrane protein with unknown function (1). TMEM106B was first identified as a major risk factor in genome-wide association studies in patients with frontotemporal lobar degeneration (FTLD), a neurodegenerative disorder characterized by atrophy in the frontal and temporal lobes of the brain (2). Localization of TMEM106B in late endosomal/lysosomal compartments suggests a role in regulating endo-lysosomal morphology and function (3). Endo-lysosomal dysfunction is likely a major cellular pathway that contributes to FTLD as other genetically linked FTLD genes, including C9orf72, GRN, and MAPT, converge on this cellular pathway (4). TMEM106B is sequentially processed through ectodomain shedding, followed by intramembrane proteolysis, with possible variability in the intramembrane cleavage site. Fragments consisting of residues 120–254 of TMEM106B have been shown to form amyloid filaments in human brains (5-7).

    Alternate Names

    FLJ11273; HLD16; MGC33727; T106B; TMEM106B; TMEM106B protein; Transmembrane protein 106B

    Database Links

    UniProt ID: Q9NUM4


    Entrez-Gene Id: 54664


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