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PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit #14119

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  • ELISA

    Supporting Data

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The rapid protocol RP PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of PSA/KLK3 in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the coated microwell plate forms a sandwich with PSA/KLK3 in a single step. has been coated onto the microwells. After incubation with cell lysates, The plate is extensively washed and TMB reagent is added for signal development. The magnitude of the absorbance for this developed color is proportional to the quantity of total PSA/KLK3. Learn more about all of your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® RP Total PSA/KLK3 Sandwich ELISA Kit detects endogenous levels of PSA/KLK3 protein. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 amino-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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