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Product last modified at: 2024-06-27T13:37:09.649Z
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PDP - Template Name: ELISA Kit
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PathScan® RP Total ULK1 Sandwich ELISA Kit #57606

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  • ELISA

    Supporting Data

    REACTIVITY H M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Total ULK1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ULK1 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with ULK1 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of ULK1. Learn more about all of your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP Total ULK1 Sandwich ELISA Kit detects endogenous levels of ULK1 protein. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene unc-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP, and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).
    1. Ogura, K. et al. (1994) Genes Dev 8, 2389-400.
    2. Kuroyanagi, H. et al. (1998) Genomics 51, 76-85.
    3. Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7.
    4. Yan, J. et al. (1999) Oncogene 18, 5850-9.
    5. Zhou, X. et al. (2007) Proc Natl Acad Sci USA 104, 5842-7.
    6. Tomoda, T. et al. (2004) Genes Dev 18, 541-58.
    7. Matsuura, A. et al. (1997) Gene 192, 245-50.
    8. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.
    9. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
    10. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
    11. Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8.
    12. Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12.
    13. Young, A.R. et al. (2006) J Cell Sci 119, 3888-900.
    14. Kamada, Y. et al. (2000) J Cell Biol 150, 1507-13.
    15. Lee, S.B. et al. (2007) EMBO Rep 8, 360-5.
    16. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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