|Product Includes||Volume||Solution Color|
|Spike Protein Coated Microwells II||96 tests|
|Anti-Human IgM, HRP-linked Antibody (ELISA Formulated)||1 ea||Red (Lyophilized)|
|Sample Diluent A 71637||25 ml|
|HRP Diluent||11 ml||Red|
|ELISA Wash Buffer (20X) 9801||25 ml|
|TMB Substrate 7004||11 ml|
|STOP Solution 7002||11 ml|
|Sealing Tape||2 ea|
|ELISA Kit #37322 Positive Control||1 ea|
|ELISA Kit #37322 Negative Control||1 ea|
NOTE: Prepare solutions with deionized/purified water or equivalent. Prepare only as much reagent as needed on the day of the experiment.
NOTE: Equilibrate all materials and prepared reagents to room temperature prior to running the assay.
Human-sourced samples should be handled in accordance with accepted safety practices. Samples should be diluted at least 1:400 with Sample Diluent A and can be further serially diluted if relative quantification is needed by the user. Positive and Negative Controls do NOT need to be diluted after reconstitution. Refer to the datasheet which shows typical results observed for the Positive Control, Negative Control, serum from uninfected individuals, and serum from SARS-CoV-2 patients. When using the cutoff criteria described below to determine if a sample is positive for anti-CoV-2 Spike Protein antibodies, samples diluted 1:400 must be compared to the undiluted Negative Control.
NOTE: Sample storage/handling, including heat-inactivation of samples, can potentially affect observed signals. Therefore, it is strongly recommended that in addition to the Positive and Negative Controls included with the kit, the user includes their own negative and positive patient samples as controls when running the assay in order to establish an appropriate cutoff value.
Add 100 µL of STOP Solution to each well. Shake gently for a few seconds.
NOTE: Initial color of positive reaction is blue, which changes to yellow upon addition of STOP Solution.
Limitations: Experimental cutoffs were determined by assaying a set of SARS-CoV-2 positive samples and uninfected donor serum collected prior to the SARS-CoV-2 pandemic.
NOTE: Positive samples were from donors with positive SARS-CoV-2 diagnosis, but IgM status for some samples may be negative due to timing of sample collection relative to disease onset.
Researchers can establish or modify this cutoff using additional samples. Positive or negative results from this assay should not be the sole basis for determining the infection status of a sample. A negative result can occur in SARS-CoV-2 patient samples due to:
posted August 2020
Protocol Id: 2224
Explore pathways + proteins related to this product.
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