PathScan® Total Btk Sandwich ELISA Kit #24547
Important Ordering Details
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Supporting Data
| REACTIVITY | H M |
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
PathScan® Total Btk Sandwich ELISA Kit detects endogenous levels of Btk protein in human or mouse cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Mouse
Background
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8).
- Khan, W.N. (2001) Immunol Res 23, 147-56.
- Lewis, C.M. et al. (2001) Curr Opin Immunol 13, 317-25.
- Salim, K. et al. (1996) EMBO J 15, 6241-50.
- Rameh, L.E. et al. (1997) J Biol Chem 272, 22059-66.
- Várnai, P. et al. (1999) J Biol Chem 274, 10983-9.
- Rawlings, D.J. et al. (1996) Science 271, 822-5.
- Park, H. et al. (1996) Immunity 4, 515-25.
- Kang, S.W. et al. (2001) EMBO J 20, 5692-702.
Limited Uses
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