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PhosphoSitePlus® Resource

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c-Kit Rabbit mAb Coated Microwells 96 tests

Product Description

CST's PathScan® Total c-Kit Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total c-Kit protein. A c-Kit Antibody has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-c-Kit proteins are captured by the coated antibody. Following extensive washing, c-Kit Mouse mAb is added to detect both the captured both phospho- and nonphospho-c-Kit proteins. Anti-mouse IgG, HRP-linked Antibody #7076* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total c-Kit protein.

* Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

CST's PathScan® Total c-Kit Sandwich ELISA Kit #7197 detects endogenous levels of total c-Kit protein. As shown in Figure 1, PathScan® Total c-Kit Sandwich ELISA Kit #7197 detects both phospho and non-phospho c-Kit protein from untreated and SCF-treated H526 cell lysates. In Figure 3, Western blot analysis of protein captured in the c-Kit Antibody #3392 coated microwell shows a single band corresponding to the c-Kit protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).


1.  Martin, F.H. et al. (1990) Cell 63, 203-11.

2.  Yarden, Y. et al. (1987) EMBO J 6, 3341-51.

3.  Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.

4.  Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.

5.  Nocka, K. et al. (1990) EMBO J 9, 1805-13.

6.  Hirota, S. et al. (1998) Science 279, 577-80.

7.  Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.


Entrez-Gene Id 3815
Swiss-Prot Acc. P10721


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7197
PathScan® Total c-Kit Sandwich ELISA Kit