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7886
PathScan® Total E-Cadherin Sandwich ELISA Kit
ELISA Kits
ELISA Kit

PathScan® Total E-Cadherin Sandwich ELISA Kit #7886

Citations (0)
PathScan® Total E-Cadherin Sandwich ELISA Kit: Image 1

Figure 1. E-cadherin is detected by PathScan® Total E-Cadherin Sandwich ELISA Kit #7886. Lysates were prepared from various cell lines and analyzed for E-cadherin using PathScan® Total E-Cadherin Sandwich ELISA Kit #7886 or by western blotting. Absorbance readings at 450 nm are shown in the top figure while the corresponding western blot using an E-cadherin antibody is shown in the bottom figure.

PathScan® Total E-Cadherin Sandwich ELISA Kit: Image 2

Figure 2. The relationship between the protein concentration of the lysate from A431 and Jurkat cells and the absorbance at 450 nm is shown.

To Purchase # 7886

Important Ordering Details

Custom Ordering Details: Product is assembled to order. Please allow up to three business days for your order to be processed.

Supporting Data

REACTIVITY

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

The PathScan® Total E-Cadherin Sandwich ELISA Kit #7886 is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of E-cadherin. An E-cadherin mouse antibody has been coated onto the microwells. After incubation with cell lysates, E-cadherin is captured by the coated antibody. Following extensive washing, an E-Cadherin rabbit detection antibody is added to detect the captured E-cadherin. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of E-cadherin.

Antibodies in kit are custom formulations specific to kit.

Specificity / Sensitivity

CST's PathScan® Total E-Cadherin Sandwich ELISA Kit #7886 detects endogenous levels of E-cadherin. As shown in Figure 1, using the Total E-Cadherin Sandwich ELISA Kit #7886, a high level of E-cadherin is detected in A431 and MCF-7 cells, but not in E-cadherin null Jurkat cells. The PathScan® Total E-Cadherin Sandwich ELISA Kit #7886 does not cross-react with human N-, P- or VE-cadherin. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

  1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
  2. Christofori, G. (2003) EMBO J. 22, 2318-2323.
  3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
  5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
  6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
  7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
  8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.