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  3. PathScan® Total iNOS Sandwich ELISA Kit

PathScan® Total iNOS Sandwich ELISA Kit #7097

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Inquiry Info. # 7097

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    Product Specifications

    REACTIVITY M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    The PathScan® Total iNOS Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of iNOS. An iNOS rabbit antibody has been coated onto the microwells. After incubation with cell lysates, iNOS protein is captured by the coated antibody. Following extensive washing, an iNOS mouse detection antibody is added to detect the captured iNOS protein. Anti-mouse IgG, HRP-linked Antibody #7076 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of iNOS.

    Protocol

    Specificity / Sensitivity

    PathScan® Total iNOS Sandwich ELISA Kit #7097 detects endogenous levels of total iNOS protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Mouse

    Background

    Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

    Alternate Names

    i-NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; Inosl; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; NOS type II; Nos-2; NOS-II; Nos2; Nos2a; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

    Database Links

    UniProt ID: P29477


    Entrez-Gene Id: 18126


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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