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7885
PathScan® Total IRS-2 Sandwich ELISA Antibody Pair
ELISA Kits

PathScan® Total IRS-2 Sandwich ELISA Antibody Pair #7885

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To Purchase # 7885

Important Ordering Details

Custom Ordering Details: Product is assembled to order. Please allow up to three business days for your order to be processed.

Supporting Data

REACTIVITY

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Product Description

CST's PathScan® Total IRS-2 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total IRS-2 Sandwich ELISA Kit #7884. Capture and detection antibodies (100X stocks) and HRP-conjugated streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an IRS-2 biotinylated rabbit detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

  1. Sun, X.J. et al. (1991) Nature 352, 73-7.
  2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
  3. Withers, D.J. et al. (1998) Nature 391, 900-904.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.