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Product Includes Volume Solution Color
MARCKS Mouse mAb Coated Microwells 96 tests
Sealing Tape 2 sheets

Product Description

The PathScan® Total MARCKS Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MARCKS protein. A MARCKS mouse antibody has been coated onto the microwells. After incubation with cell lysates, MARCKS (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a MARCKS rabbit detection antibody is added to detect captured MARCKS protein. Anti-Rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total MARCKS protein.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

PathScan® Total MARCKS Sandwich ELISA Kit #7188 detects endogenous levels of MARCKS protein as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).


1.  Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.

2.  Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.

3.  Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.

4.  Hartwig, J.H. et al. (1992) Nature 356, 618-22.

5.  Thelen, M. et al. (1991) Nature 351, 320-2.


Entrez-Gene Id 4082
Swiss-Prot Acc. P29966


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7188
PathScan® Total MARCKS Sandwich ELISA Kit