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XML generation date: 2026-04-13 20:05:16.984
Product last modified at: 2025-11-10T08:01:18.363Z
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PDP - Template Name: ELISA Kit
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  1. Products /
  2. ELISA Kits /
  3. PathScan® Total MARCKS Sandwich ELISA Kit

PathScan® Total MARCKS Sandwich ELISA Kit #7188

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Inquiry Info. # 7188

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The PathScan® Total MARCKS Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MARCKS protein. A MARCKS mouse antibody has been coated onto the microwells. After incubation with cell lysates, MARCKS (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a MARCKS rabbit detection antibody is added to detect captured MARCKS protein. Anti-Rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total MARCKS protein.
    Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Total MARCKS Sandwich ELISA Kit #7188 detects endogenous levels of MARCKS protein as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

    Alternate Names

    80K-L; 80K-L protein; FLJ14368; FLJ90045; MACS; MARCKS; MARCS; myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich protein kinase C substrate; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); phosphomyristin; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain

    Database Links

    UniProt ID: P29966


    Entrez-Gene Id: 4082


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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