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  3. PathScan® Total p27 Kip1 Sandwich ELISA Kit

PathScan® Total p27 Kip1 Sandwich ELISA Kit #7768

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Inquiry Info. # 7768

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    Product Specifications

    REACTIVITY H M
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    The PathScan® Total p27 Kip1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of p27 Kip1. A p27 Kip1 Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, p27 Kip1 protein is captured by the coated antibody. Following extensive washing, a p27 Kip1 Mouse Detection Antibody is added to detect the captured p27 Kip1 protein. Anti-Mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of p27 Kip1 protein.
    Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Total p27 Kip1 Sandwich ELISA Kit detects endogenous levels of total p27 Kip1 protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

    Alternate Names

    CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; KIP1; MEN1B; MEN4; p27Kip1

    Database Links

    UniProt ID: P46527


    Entrez-Gene Id: 1027


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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