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PhosphoSitePlus® Resource

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REACTIVITY
Product Includes Volume Solution Color
Src Rabbit mAb Coated Microwells 96 tests
Sealing Tape 2 sheets

Product Description

The PathScan® Total Src Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total Src protein. A Src rabbit antibody has been coated onto the microwells. After incubation with cell lysates, Src protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Src mouse detection antibody is added to detect the captured Src protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. The HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total Src protein.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

The PathScan® Total Src Sandwich ELISA Kit #7984 from Cell Signaling Technology detects endogenous levels of total Src protein, as shown in Figure 1. This kit does not cross-react with the Src family members Yes, Lck, Lyn or Hck. Other family members have not been tested. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).


1.  Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.

2.  Hunter, T. (1987) Cell 49, 1-4.


Entrez-Gene Id 6714
Swiss-Prot Acc. P12931


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7984
PathScan® Total Src Sandwich ELISA Kit