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51367S 100 µl (10 western blots) $112.00.0
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PhosphoSitePlus® Resource

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Product Description

Nonphosphorylated 4E-BP1 Control Cell Extracts: Total cell extracts from MCF7 cells, amino acids starved for 1 hour to serve as a negative control. Supplied in SDS Sample Buffer.

Phosphorylated 4E-BP1 Control Cell Extracts: Total cell extracts from MCF7 cells, amino acids starved for 1 hour followed by adding back amino acids for 1 hour and treating with 100 nM insulin for 30 min to serve as a positive control. Supplied in SDS Sample Buffer.


Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).


1.  Pause, A. et al. (1994) Nature 371, 762-7.

2.  Brunn, G.J. et al. (1997) Science 277, 99-101.

3.  Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.

4.  Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.

5.  Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.



For Research Use Only. Not For Use In Diagnostic Procedures.
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51367
4E-BP1 Control Cell Extracts