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Render Timestamp: 2024-12-06T11:37:56.646Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-20 06:21:06.539
Product last modified at: 2024-12-05T00:45:07.543Z
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PDP - Template Name: Protein Control Kit
PDP - Template ID: *******5c6809e

Biotinylated Protein Ladder Detection Pack #7727

    Product Information

    Product Usage Information

    (a) Thaw protein ladder on ice. Mix well and aliquot the desired amount of the protein ladder (10 µl for mini-gels and 20 µl for full length gels) into a separate tube.

    (b) Heat the ladder to 95-100ºC for 2 minutes.

    (c) After a quick microcentrifuge spin, load directly onto gel. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.

    (d) Peform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot, concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000. Incubate for 1 hour at room temperature with gentle agitation.

    (e) Proceed as usual with the detection procedure

    Storage

    Biotinylated Protein Ladder: Store at -20°C.
    Anti-biotin HRP-linked Antibody: Store at -20°C.

    Product Description

    Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on Western blots when using the horseradish peroxidase (HRP) based Western detection system. The pack contains Biotinylated Protein Ladder and Anti-biotin, HRP-linked Antibody. The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa. The anti-biotin antibody is used to detect biotinylated protein ladders on Western blots. The pack is optimized for chemiluminescent Western detection procedures.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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