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7727
Biotinylated Protein Ladder Detection Pack
Experimental Controls
MW Marker Kit

Biotinylated Protein Ladder Detection Pack #7727

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Biotinylated Protein Ladder Detection Pack: Image 1

To Purchase # 7727S
Product # Size Price
7727S
650 µl $ 66
7727L
3250 µl $ 235

Product Usage Information

(a) Thaw protein ladder on ice. Mix well and aliquot the desired amount of the protein ladder (10 µl for mini-gels and 20 µl for full length gels) into a separate tube.

(b) Heat the ladder to 95-100ºC for 2 minutes.

(c) After a quick microcentrifuge spin, load directly onto gel. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.

(d) Peform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot, concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000. Incubate for 1 hour at room temperature with gentle agitation.

(e) Proceed as usual with the detection procedure

Storage

Biotinylated Protein Ladder: Store at -20°C.Anti-biotin HRP-linked Antibody: Store at -20°C.

Product Description

Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on Western blots when using the horseradish peroxidase (HRP) based Western detection system. The pack contains Biotinylated Protein Ladder and Anti-biotin, HRP-linked Antibody. The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa. The anti-biotin antibody is used to detect biotinylated protein ladders on Western blots. The pack is optimized for chemiluminescent Western detection procedures.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.