c-Jun Fusion Protein Beads selectively "pull down" SAPK/JNK and also serve as a useful substrate to measure SAPK/JNK activity. It is expressed as a recombinant protein fusion to amino acid residues corresponding to c-Jun codons 1-89. The fusion protein is produced in E. coli and is supplied as a 50% slurry of c-Jun Fusion Protein beads.
Prior to use, put the tube on ice for 5 minutes to lower viscosity of buffer. Then beads should be resuspended to a 50% slurry by inversion or gentle vortexing.
20 mM Tris-HCl (pH 7.4)
150 mM NaCl
1 mM Na2EDTA
1 mM EGTA
1% Triton X-100
2.5 mM sodium pyrophosphate
1 mM β-glycerophosphate
1 mM Na3VO4
1 µg/ml leupeptin
Note: Please aliquot prior to first use.
Store at -20°C.
c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines, and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).
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