Boil for 3 minutes prior to use. Load 10 µl of phosphorylated and nonphosphorylated CREB Control Cell Extracts per lane.
Supplied in SDS Sample Buffer: 62.5 mM Tris-HCL (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C.
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