GSK-3 Fusion Protein

Inquiry Info. # 9237

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Product Specifications

MW (kDa)

27

Product Information

Product Description

GSK-3 Fusion Protein serves as a useful substrate for assaying Akt kinase activity. It is expressed as a protein fusion to the GSK-3 α/β crosstide, corresponding to residues surrounding GSK-3 α/β (Ser21/9) (CGPKGPGRRGRRRTSSFAEG).

MW (kDa)

27

Storage

Store at -20°C.

Source / Purification

GSK-3 Fusion Protein is expressed as a GST fusion protein in E. coli.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Alternate Names

Glycogen Synthase Kinase; gsk-3α; gsk-3β; GSK3; gsk3α; gsk3β; pp110; screeningkit; substrate

Database Links

UniProt ID: P49840 , P49841

Entrez-Gene Id: 2931 , 2932

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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GSK-3 Fusion Protein #9237