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  3. Human CD3 zeta (R52-R164)-3xG4S Recombinant Protein Quantitative Control

Human CD3 zeta (R52-R164)-3xG4S Recombinant Protein Quantitative Control #25884

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  • ELISA

    Product Specifications

    MW (kDa) 28
    Application Key:
    • ELISA-ELISA 

    Product Information

    Product Usage Information

    Prior to use, thaw the recombinant protein and briefly spin down to collect all liquid at the bottom of the tube. Dilute according to the following instructions depending on the ELISA kit type. An example sensitivity curve is shown in Figure 2.

    PathScan® ELISA Kits:
    Dilute with ELISA Sample Diluent to achieve a 1X protein concentration.

    PathScan® RP ELISA Kits:
    Dilute with 1X Cell Lysis Buffer #9803 to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the detection antibody.

    FastScan™ ELISA Kits:
    Dilute with 1X FastScan™ ELISA Cell Extraction Buffer to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the antibody cocktail.

    Formulation

    Expression Host: 293

    Supplied in 1X PBS (8.1mM Na2HPO4, 2.68 mM KCl, 1.675 mM KH2PO4, and 137 mM NaCl (pH 7.4)).

    Storage

    Store at -20°C.

    Product Description

    Human CD3ζ (R52-R164)-3xG4S Recombinant Protein Quantitative Control is derived from a recombinant expression construct with CD3ζ (R52-R164) and 15-mer (G4S)3. The protein can be used with PathScan® RP G4S Linker/CD3ζ CAR Sandwich ELISA Kit #20418 and G4S Linker/CD3ζ CAR Matched Antibody Pair #81909 as a quantitative control. Suitable for use in other G4S Linker/CD3ζ assays when a control is needed.
    MW (kDa) 28
    Purity Assessed to be >93% by densitometry using SDS-PAGE analysis and Coomassie Blue detection.

    Background

    The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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