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Product Description

This peptide is used to block Jak1 (6G4) Rabbit mAb #3344 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Jak1 (6G4) Rabbit mAb #3344 by dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.


1.  Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.

2.  Darnell, J.E. (1997) Science 277, 1630-5.

3.  VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.

4.  Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.

5.  Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.


Entrez-Gene Id 3716
Swiss-Prot Acc. P23458


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Jak1 Blocking Peptide