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|9160S||200 µl (10 western blots)||$112.00.0|
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MEK1/2 Control Cell Extracts #9160
Nonphosporylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight serve as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight then treated with 200 nM TPA #4174 for 15 minutes to serve as a positive control. Supplied in SDS Sample Buffer.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
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