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Product Description

This peptide is used to specifically block mTOR (7C10) Rabbit mAb #2983 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks mTOR (7C10) Rabbit mAb #2983 signal in dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot and Western immunoblotting protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).


No product specific background information is currently available for this product.


1.  Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.

2.  Brown, E.J. et al. (1994) Nature 369, 756-8.

3.  Sabatini, D.M. et al. (1994) Cell 78, 35-43.

4.  Gingras, A. C. et al. (2001) Genes and Develop. 15, 807-826.

5.  Dennis, P.B. et al. (2001) Science 294, 1102-5.

6.  Fang, Y. et al. (2001) Science 294, 1942-5.

7.  Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.

8.  Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.

9.  Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.


Entrez-Gene Id 2475
Swiss-Prot Acc. P42345


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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mTOR Blocking Peptide