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41645
NRF2 Control Cell Extracts
Experimental Controls
Cell Extract Kit

NRF2 Control Cell Extracts #41645

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  1. WB
Western Blotting Image 1: NRF2 Control Cell Extracts
Western blot analysis of NRF2 Control Cell Extracts using NRF2 (D1Z9C) XP® Rabbit mAb #12721 (left, upper) and NRF2 (E5F1A) Rabbit mAb #20733 (right, upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Product Usage Information

Boil for 3 minutes prior to use. Load 10 µL of untreated and MG-132 treated NRF2 Control Cell Extracts per lane.

Storage

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C, or at –80°C for long-term storage.

Product Description

NRF2 Control Cell Extracts (A-172 untreated): Total cell extracts from A-172 cells serve as a negative control. Supplied in SDS Sample Buffer.

NRF2 Control Cell Extracts (A-172 + MG-132): Total cell extracts from A-172 cells treated with MG-132 (10 μM, overnight) serve as a positive control.

This lysate pair is produced as a control for western blotting of NRF2 protein.

Background

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).
  1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
  2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
  3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
  4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
  5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 41645
Cat. # Size Qty. Price
41645S
100 µl  (10 western blots)
$ 132