p44/42 MAPK (Erk1/2) Control Cell Extracts #9194
- WB
Product Information
Product Usage Information
Boil for 3 minutes prior to use. Load 15 μl of nonphosphorylated and phosphorylated p44/42 MAPK (Erk1/2) Control Cell Extracts per lane.
Storage
(pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT,
0.01% w/v bromophenol blue or phenol red.
Store at –20°C or –80°C for long-term storage.
Product Description
Phosphorylated p44/42 MAPK (Erk1/2) Control Cell Extracts: Total cell extracts from Jurkat cells treated with TPA #4174 at 200 nM for 20 minutes, to serve as a positive control. Supplied in SDS Sample Buffer.
Background
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
Alternate Names
control lysate; erk; erk1; Erk1/2; erk2; jurkat; map kinase; mapk; negative control; p42; p44; p44/42; phorbol ester; pma; positive control; tpa; u0126
Limited Uses
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