This biotinylated peptide contains the residues surrounding Ser133 of CREB. The serine residue in the peptide corresponding to Ser133 has been chemically phosphorylated in the course of peptide synthesis. This phosphopeptide was generated for use as a positive control in kinase assays (PKCα Kinase #7578, PKCβ I Kinase #7581, PKCβ II Kinase #7584, PKCγ Kinase #7596, PKCδ Kinase #7342, PKCε Kinase #7492, PKCζ Kinase #7608, PKCη Kinase #7593, PKCθ Kinase #7605, PKCι Kinase #7599, PKCμ Kinase #7602), but it may also serve as a positive control in other heterogeneous or homogeneous kinase assays.
This phosphorylated peptide can be detected with the Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624. Sample kinase assay protocol is attached.
Supplied in 0.0001% DMSO. Store at -20°C.
The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.
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