This biotinylated peptide contains the residues surrounding Ser1177 of eNOS. The serine residue in the peptide has been chemically phosphorylated in the course of peptide synthesis. This peptide was generated for use as a substrate in heterogeneous or homogeneous kinase assays.
This phosphorylated peptide can be detected with the Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570. A sample kinase assay protocol is attached.
The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.
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