Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide #1047
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Important Ordering Details
Custom Ordering Details: This product is assembled upon order. Please allow two-four weeks for your product to be processed.Product Information
Product Usage Information
Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.
Storage
Product Description
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149 by dot blot.
Background
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
- Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
- Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
- Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
- Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
- Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
- Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.
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