Render Target: STATIC
Render Timestamp: 2024-10-14T09:56:45.845Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-04-05 20:22:12.712
Product last modified at: 2024-06-27T13:36:13.431Z
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PDP - Template Name: Blocking Peptide
PDP - Template ID: *******6db2f4c

Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide #1023

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    Product Information

    Product Usage Information

    Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot and Western immunoblotting protocols.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

    Product Description

    This peptide is used to block Phospho-IKK-a/b (Ser176/180) (16A6) Rabbit mAb #2697 reactivity.

    Quality Control

    The quality of the peptide was evaluated by reverse-phase HPLC and by mass spectrometry. The peptide blocks Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb #2697 signal in dot blot.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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