Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (#3007) in the presence of control peptide (left), or Phospho-MAPKAPK-2 (Thr334) Blocking Peptide (right).
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.
Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.
This peptide is used to block Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 reactivity. It can also be used to block Phospho-MAPKAPK-2 (Thr334) Antibody #3041.
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 signal in immunohistochemistry.
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
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