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Phospho-MAPKAPK-2 (Thr334) Blocking Peptide

Phospho-MAPKAPK-2 (Thr334) Blocking Peptide #1041

Inquiry Info. # 1041

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Product Information

Product Usage Information

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

Storage

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Product Description

This peptide is used to block Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 reactivity. It can also be used to block Phospho-MAPKAPK-2 (Thr334) Antibody #3041.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 signal in immunohistochemistry.

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

Alternate Names

map; map-kinase; mapk; mapkap; mapkap 2; mapkap2; mapkapk 2; mapkapk-2; mapkapk2; mk 2; mk2; mpakap-2

Database Links

UniProt ID: P49137

Entrez-Gene Id: 9261

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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