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Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 in the presence of control peptide (left) or Phospho-Stat1 (Tyr701) Blocking Peptide (right).

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Product Description

This peptide is used to block Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 signal in dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.


1.  Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.

2.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

3.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

4.  Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.

5.  Frank, D.A. (1999) Mol Med 5, 432-56.

6.  Wen, Z. et al. (1995) Cell 82, 241-50.


Entrez-Gene Id 6772
Swiss-Prot Acc. P42224


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Phospho-Stat1 (Tyr701) Blocking Peptide