Upstream / Downstream
Explore pathways related to this product.
Our U.S. Offices Are Closed
Our U.S. offices are closed in observance of Memorial Day. We will reopen on Tuesday, May 30th.
Thank you for your patience.
To Purchase # 9303S
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
Rb Control Proteins #9303
Western blot analysis of Rb-C Fusion Protein #6022 (amino acids 701-928 of Rb fused to MBP) before (-) and after (+) in vitro phosphorylation by cdc2/cyclin B Protein Kinase (New England Biolabs #P6020), using Phospho-Rb (Ser795) Antibody #9301 (left) or the control antibody (right).Learn more about how we get our images
Gallery: Rb Control Proteins #9303
Nonphosphorylated Rb-C Fusion Protein (5 µg/ml): Rb-C is expressed as a recombinant fusion protein of Rb residues 701–928 and maltose binding protein, serves as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated Rb-C Fusion Protein (5 µg/ml): Prepared by in vitro kinase reaction with cdc2, serves as a positive control. Supplied in SDS Sample Buffer.Molecular Formula:
Apparent Molecular Weight: Both the nonphosphorylated and phosphorylated forms of Rb-C migrate at an apparent molecular weight of 76 kDa by SDS-PAGE.
These lysates are useful controls for total and Phospho-Rb antibodies. However, this truncated Rb recombinant protein is not recognized by Phospho-Rb (Ser608) Antibody #2181, Phospho-Rb (Ser608) (D10F2) RmAb #8147, or Rb (D20) Rabbit mAb #9313.Storage: Store at -20°C.
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.