ELISA analysis of PathScan® Sandwich ELISA Control Phospho Cell Extracts I using multiple PathScan® Sandwich ELISA kits. Samples were prepared using the standard ELISA protocol (attached) to a final concentration of 0.25 mg/ml and assayed using the indicated ELISA kits.
Supplied as lyophilized product. Keep at -20°C for short term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles.
Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.
PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts I can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.