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7989
PathScan® Sandwich ELISA Control Phospho Cell Extract II
Experimental Controls

PathScan® Sandwich ELISA Control Phospho Cell Extract II #7989

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ELISA analysis of PathScan® Sandwich ELISA Control Phospho Cell Extracts II using multiple PathScan® Sandwich ELISA kits. Samples were prepared using the standard ELISA protocol (attached) to a final concentration of 1.0 mg/ml and assayed using the indicated ELISA kits.

Storage:

Supplied as lyophilized product. Keep at -20°C for short-term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles.

Product Description

Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.

Background

PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts II can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

To Purchase # 7989S
Product # Size Price
7989S
1 vial  (12 assays) $ 97