News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
Important Ordering DetailsCustom Ordering Details: Sections are cut freshly upon ordering. Please allow up to three business days for your product to be processed.
Research More. Spend Less.
Spend $650 or more and get 20% off*
(*Offer valid in the US only. Expires June 30, 2017)
To Purchase # 13752S
|13752S||1 Pack (5 slides)||$132.00.0|
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
SignalSlide® LKB1 IHC Controls #13752
Gallery: SignalSlide® LKB1 IHC Controls #13752
- Immunohistochemical analysis of paraffin-embedded LNCaP (left) and A549 (right) cell pellets using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) #13031.
Each control slide contains formalin fixed, paraffin-embedded cell pellets, LNCaP (LKB1 positive) and A549 (LKB1 negative), that serve as a control for LKB1 immunostaining.
LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).
Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalSlide is a registered trademark of Cell Signaling Technology, Inc.